Expression, Purification, Characterization and Protein Catalysis of mutated Fluorescent Proteins
Internship Description
Workshop Description
A fluorescent protein variant was tailor-made as
a suitable host for the incorporation of artificial
metal centers through in silico removal of metal binding motifs and
improvement of thermal, salt and solvent stability.
Through introduction of mutations, metalloproteins are generated, which posses reactivities nature does not provide. These
metalloproteins will be tested in aqueous catalysis reactions and the influence
of mutations on selectivity and
reactivity will be studied.
Deliverables/Expectations
Deliverables
Students shall
extend their general knowledge and
skills in molecular biology and
protein biochemistry. An emphasis will be put on expression, purification
and characterization techniques. If
capable mutant proteins will be crystallized
to determine structural properties. Students will be taught to work independently on
projects, yet
strengthening their
critical sense to develop new ideas. In the course of the internship students shall demonstrate this understanding
during oral resentations and one
final written report.
Faculty Name
Magnus Rueping
Field of Study
Field Of Study
Chemistry/Biochemistry/Molecular Biology