During multicellular
development, the specification of distinct cell fates is often regulated by the
same transcription factors operating differently in distinct cis-regulatory
modules. In the model plant Arabidopsis root, distinct cell types are determined
by the interacting transcription factors within one contiguous tissue layer.
Using fluorescence lifetime microscopy we reveal a cell type-specific protein complexes that differentially
regulate target genes and instruct distinct cell fates. In addition to
determining the network function in other organs, we are currently studying how
are these complexes established and maintained by generating a temporal
interaction map and dissecting the transcriptional regulation by these
complexes.We will use the following tools: FRET-FLIM, confocal
microscopy imaging, mutants analysis.