Micritization under elevated temperature and pressure

Micritization under elevated temperature and pressure

Internship Description

Microrhombic calcite crystals, which form the frame of microporosity in most Middle Eastern carbonate reservoirs, formed at temperatures of 60°C
to 80°C and a burial depth in excess of 1km (Vahrenkamp et al., 2014; Swart et al., 2016). Since there are possibilities by some initial experiments
on the ability of microbes to be alive and perform diagenetic alterations at elevated temperatures and pressure (Daffonchio and co-workers,
unpublished data; see preliminary data below), we propose to investigate whether microbes are involved in the generation of those microrhombic
crystals through microbial diagenesis of previous micritized sediments.
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Deliverables/Expectations

​• Micritization experiments will be simulated at burial diagenetic conditions
of high hydrostatic pressure (HP: 0.1 - 25 MPa) and temperature (T: 10 -
70°C) in bioreactors
• The activity of several bacterial strains from pure and mixed culture
collections (e.g., Escherichia coli) and/or those obtained from the
sediments in Task 3.2 will be tested at different conditions of HP.
• Growth of cells will be measured by bacterial counts after fluorescence in
situ hybridization (FISH) staining coupled with flow cytometry.

Faculty Name

Volker Vahrenkamp

Field of Study

​Geomicrobiology